What is 50X TAE Buffer Tris-Acetate-EDTA used for?

50X TAE Buffer Tris-Acetate-EDTA is primarily used in molecular biology for nucleic acid electrophoresis. This concentrated buffer allows for efficient separation of DNA and RNA in gel electrophoresis techniques. When diluted, it maintains the pH and ionic environment necessary for accurate results during DNA analysis, cloning, and other genetic applications.

How do you prepare 1X TAE Buffer from 50X concentration?

To prepare 1X TAE Buffer from 50X concentration, dilute the buffer by mixing 1 part of 50X TAE with 49 parts of distilled water. This results in a final volume where the buffer is at the appropriate dilution for running gel electrophoresis, ensuring the components are balanced for optimal performance in your experiments.

Why is Tris-Acetate-EDTA preferred over other buffers?

Tris-Acetate-EDTA (TAE) is favored for its ability to provide consistent buffering capacity and low electroendosmosis during electrophoresis. Unlike other buffers, TAE allows for the recovery of nucleic acids post-electrophoresis as it does not interfere with downstream applications, making it ideal for both analysis and cloning of DNA.

Can TAE Buffer be reused after gel electrophoresis?

Generally, TAE Buffer may be reused if it remains clean and free from contaminants. However, repeated use can compromise the buffer's pH and ionic strength, affecting the results of subsequent experiments. Always evaluate the quality of the buffer after each use and consider preparing fresh buffer for critical applications to maintain accuracy.

What are the storage conditions for 50X TAE Buffer?

50X TAE Buffer should be stored in a cool, dark place, ideally at room temperature or in a refrigerator to maintain its efficacy. Properly sealing the bottle after use will prevent contamination. If stored correctly, it can remain stable for an extended period, ensuring it's ready for your experimental needs.

Is 50X TAE Buffer compatible with all types of gel electrophoresis?

Yes, 50X TAE Buffer is compatible with various gel electrophoresis methods, including agarose and polyacrylamide gels. While TAE is typically used for DNA applications, it's important to adapt your experiments based on the specific type of nucleic acids being analyzed to achieve the best results.

How does the concentration of TAE Buffer impact electrophoresis results?

The concentration of TAE Buffer used directly impacts the resolution and migration speed of nucleic acids during electrophoresis. Higher concentrations can lead to slower migration and better resolution in separating fragments, while lower concentrations may generate faster migration, making it necessary to optimize based on the experiment's needs.

What pH does 50X TAE Buffer maintain?

50X TAE Buffer typically maintains a pH around 8.0, which is optimal for most enzymatic reactions and electrophoresis processes. This pH ensures stability and functionality, providing a conducive environment for nucleic acid interactions and separation during experimental procedures in molecular biology.

Can I use 50X TAE Buffer for RNA electrophoresis?

Yes, 50X TAE Buffer can be used for RNA electrophoresis, although many prefer using 1X TBE for RNA as it offers sharper bands. However, TAE still effectively provides a stable environment for RNA separation, making it a suitable choice in many laboratory applications involving RNA.

Where can I buy 50X TAE Buffer Tris-Acetate-EDTA, 100 mL in Malaysia?

You can purchase 50X TAE Buffer Tris-Acetate-EDTA, 100 mL from Ubuy, which offers a wide selection of laboratory supplies that can be delivered to your location in Malaysia. Ubuy ensures that you receive a product that meets your experimental requirements.

Bagaimana untuk Membeli-belah 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL Dalam Talian Dari Ubuy?

Sangat mudah untuk membeli-belah 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL dalam talian daripada Ubuy.. Anda hanya perlu mencari produk, pilih kaedah penghantaran anda semasa mendaftar keluar dan hantar ke lokasi anda.

Adakah 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL Tersedia untuk Beli-belah Dalam Talian dalam Malaysia?

Ya, di Ubuy Malaysia produk ini tersedia untuk anda membeli-belah pada harga yang berpatutan.. 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL tidak tersedia secara tempatan tetapi anda boleh mempercayai kami dengan perkhidmatan penghantaran ekspres kami.

Berapa lama masa yang diperlukan untuk mendapatkan produk selepas membuat pesanan?

Masa penghantaran produk pesanan anda berbeza mengikut apa yang anda pesan dan kaedah penghantaran yang anda pilih.. Anggaran masa penghantaran dinyatakan semasa proses daftar keluar, jadi berhati-hati semasa membeli-belah.

eCon Lab Supply Lihat Asal

50X TAE Buffer (Tris-Acetate-EDTA), 100 mL

50X TAE Buffer is used for running electrophoresis of nucleic acids in gels, providing electrical conductivity and maintaining pH, while protecting DNA from nucleases.

eCon Lab Supply

Item #: 85519151

50X TAE Buffer (Tris-Acetate-EDTA), 100 mL

Item #: 85519151

MYR 223

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from AS}

Ketersediaan : Dalam stok

Diimport dari kedai USA

QTY:

Pesan sekarang dan dapatkannya lebih kurang pada Rabu, September 02

Secured transaction

Our Top Logistics Partners

50X TAE Buffer is used for running electrophoresis of nucleic acids in gels, providing electrical conductivity and maintaining pH, while protecting DNA from nucleases.

Waranti U-Care:

Tiada

Pilih pelan

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of AS store (110-120). Recommended power converters Beli Sekarang.

What Stands Out

High Purity

Formulated with high-quality components, this TAE buffer ensures reliable results in your molecular biology experiments, minimizing contamination risks and enhancing reproducibility in gel electrophoresis.

Convenient Size

The 100 mL volume is ideal for lab use, providing ample buffer for multiple experiments while reducing waste, enabling efficient resource management for researchers in DNA and RNA analysis.

Ready-to-Use

This pre-prepared 50X TAE buffer allows for immediate application in your procedures, saving time in preparation and ensuring that your experiments can proceed without unnecessary delays.

Maklumat produk

Container Type	Bottle
Item Form	Gel
Item Weight	4 ounces (113.4 grams)
Volume	100 Milliliters
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Part Number	23.105.17
Included Components	Bottle
Brand	eCon Lab Supply
Item Volume	100 Milliliters
Package Information	Bottle

Who Should Buy?

Molecular Biologists
Ideal for DNA electrophoresis, providing efficient buffering capacity for nucleic acid analysis in lab experiments.
Academic Institutions
Supports educational labs where students learn about genetic analysis, making it a staple in biology curricula.
Research Facilities
Highly suitable for ongoing research projects involving DNA and RNA studies, thanks to its reliable buffering properties.

Non-Laboratory Use
Not designed for applications outside of laboratory settings; unsuitable for general household or consumer use.

PENERANGAN PRODUK

50X TAE Buffer (Tris-Acetate-EDTA), 100 mL

Ada sebarang Pertanyaan? Berbual dengan kami

Soalan & Jawapan Pelanggan

soalan: Bagaimana untuk Membeli-belah 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL Dalam Talian Dari Ubuy?

jawab: Sangat mudah untuk membeli-belah 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL dalam talian daripada Ubuy.. Anda hanya perlu mencari produk, pilih kaedah penghantaran anda semasa mendaftar keluar dan hantar ke lokasi anda.
soalan: Adakah 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL Tersedia untuk Beli-belah Dalam Talian dalam Malaysia?

jawab: Ya, di Ubuy Malaysia produk ini tersedia untuk anda membeli-belah pada harga yang berpatutan.. 50X TAE Buffer (Tris-Acetate-EDTA), 100 mL tidak tersedia secara tempatan tetapi anda boleh mempercayai kami dengan perkhidmatan penghantaran ekspres kami.
soalan: Berapa lama masa yang diperlukan untuk mendapatkan produk selepas membuat pesanan?

jawab: Masa penghantaran produk pesanan anda berbeza mengikut apa yang anda pesan dan kaedah penghantaran yang anda pilih.. Anggaran masa penghantaran dinyatakan semasa proses daftar keluar, jadi berhati-hati semasa membeli-belah.

eCon Lab Supply Buffers Editorial Review

Tiada ulasan editorial ditemui

Customer Reviews & Ratings

5.0

1 rating pelanggan

5 bintang

100%
4 bintang

0%
3 bintang

0%
2 bintang

0%
1 bintang

0%

Ulas Produk Ini

Kongsikan pandangan anda dengan pelanggan lain

Kebaikan

High-quality buffer solution
Stable and reliable performance
Easy to use in experiments
Excellent for electrophoresis
Great customer reviews

Keburukan

Some may find the size too small.

Product Price History

Maklumat penting

Pengehadan: Untuk produk penghantaran antarabangsa, harap maklum bahawa sebarang jaminan pengeluar mungkin tidak sah; pilihan perkhidmatan pengeluar mungkin tidak tersedia; manual produk, arahan, dan amaran keselamatan mungkin tidak dalam bahasa negara destinasi; produk-produk (dan bahan-bahan berkaitannya) mungkin tidak direka selaras dengan standard negara destinasi, spesifikasi, dan syarat pelabelan; dan produk-produk itu mungkin tidak mematuhi kepada voltan negara destinasi dan lain-lain standard elektrik (memerlukan penggunaan adaptor atau penukar aliran jika perlu). Penerima adalah bertanggungjawab untuk memastikan bahawa produk itu boleh diimport selaras dengan undang-undang negara destinasi. Apabila memesan dari Ubuy atau pihak afiliasinya, si penerima adalah merupakan pengimport dalam rekod dan mesti mematuhi kepada semua undang-undang dan peraturan negara destinasi.
Bukan semua produk yang tersenarai di Ubuy adalah untuk dijual, kerana Ubuy adalah enjin carian global. Produk-produk adalah tertakluk kepada peraturan eksport/perdagangan.

MYR 223

Pesan sekarang dan dapatkannya lebih kurang pada Rabu, September 02

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

Ciri & Faedah

Ideal for running electrophoresis of nucleic acids
Provides electrical conductivity and maintains pH
Protects DNA from nucleases
Suitable for both genomic and large supercoiled DNA
Can be used as a gel preparation buffer

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

50X TAE Buffer (Tris-Acetate-EDTA), 100 mL

MYR 223

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Maklumat produk

Who Should Buy?

PENERANGAN PRODUK

Soalan & Jawapan Pelanggan

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

Ulas Produk Ini

Kebaikan

Keburukan

Product Price History

Maklumat penting

MYR 223

Ciri & Faedah

Ubuy Assurance

Fast
Shipping

Free
Return^*