What is 50X TAE Buffer Tris-Acetate-EDTA used for?

50X TAE Buffer Tris-Acetate-EDTA is commonly used in molecular biology applications, particularly for DNA and RNA gel electrophoresis. This buffer maintains a stable pH and optimal ionic strength, facilitating efficient separation and visualization of nucleic acids during electrophoresis. Researchers utilize this buffer for preparing agarose gels and running electrophoresis to analyze DNA fragments, test RNA quality, or conduct DNA sequencing.

How do I prepare working solutions from 50X TAE Buffer?

To prepare a working solution from 50X TAE Buffer, dilute it with distilled water. For a standard 1X working solution, combine 1 part of 50X TAE Buffer with 49 parts of distilled water, resulting in a total of 50 mL. This dilution is crucial for achieving the proper ionic concentration for successful electrophoresis. This process is applicable for various laboratory setups where precise measurements of nucleic acids are needed.

Can I store 50X TAE Buffer long-term?

Yes, 50X TAE Buffer can be stored long-term if kept at appropriate conditions. For optimal stability, store it in a cool, dark place, preferably at room temperature, or refrigerate it. However, avoid freezing the buffer as it can affect the buffer's performance. Proper storage ensures its effectiveness in various applications, such as DNA extraction and analysis, allowing researchers to maintain a consistent workflow over time.

Is 50X TAE Buffer suitable for RNA electrophoresis?

Yes, 50X TAE Buffer is suitable for RNA electrophoresis as it provides an effective medium for separation. The buffer’s ion composition aids in the resolution of RNA molecules, allowing for clearer results during gel electrophoresis. Additionally, it ensures that RNA maintains its structural integrity, making it ideal for applications in gene expression studies and other molecular biology experiments involving RNA.

What volume of 50X TAE Buffer do I need for a standard gel?

For a standard gel, the volume of 50X TAE Buffer needed will depend on the total volume of gel you wish to prepare. Typically, for a 100 mL agarose gel, you would use 2 mL of 50X TAE Buffer and add 98 mL of distilled water. This ensures that the final buffer concentration in the gel is at 1X, allowing for effective nucleic acid separation during electrophoresis.

Can 50X TAE Buffer be reused after gel electrophoresis?

While technically possible, reusing 50X TAE Buffer after gel electrophoresis is not recommended due to potential contamination and changes in ionic concentration. Each use can introduce impurities that may skew results in subsequent experiments. Fresh buffer ensures consistent performance, especially for critical applications like cloning and sequencing where precision is essential.

Does 50X TAE Buffer require pH adjustment?

50X TAE Buffer is generally prepared at a neutral pH of approximately 8.0 and does not require further adjustment when used directly from the bottle. If diluting for specific applications, it’s advisable to verify the pH, especially in sensitive experiments where pH can impact nucleic acid stability. Maintaining the correct pH is essential for optimal results in electrophoresis.

What are the alternatives to 50X TAE Buffer?

Alternatives to 50X TAE Buffer include TBE (Tris-Borate-EDTA) Buffer and others like SSC (Saline-Sodium Citrate) Buffer. Each buffer type has its unique advantages; for instance, TBE is often used for high-resolution separation of DNA fragments. Researchers may choose based on factors such as the type of nucleic acids being analyzed or the specific requirements of their experiment.

How does 50X TAE Buffer compare to 1X TAE Buffer?

50X TAE Buffer is a concentrated form, while 1X TAE Buffer is the diluted, working solution used for electrophoresis. The 50X concentration allows for greater convenience, as only a small amount is required to prepare a larger volume of 1X buffer. This concentration flexibility is beneficial for laboratories needing to conserve space or resources while preparing buffer solutions for multiple experiments.

Where can I buy 50X TAE Buffer Tris-Acetate-EDTA, 1L in Malaysia?

You can purchase 50X TAE Buffer Tris-Acetate-EDTA, 1L at Ubuy. Ubuy offers a comprehensive selection of laboratory reagents and supplies, making it a convenient choice for researchers looking for quality products. Their user-friendly interface enables easy finding and ordering of essential lab materials, ensuring you have what you need for your experiments.

Bagaimana untuk Membeli-belah 50X TAE Buffer (Tris-Acetate-EDTA), 1L Dalam Talian Dari Ubuy?

Sangat mudah untuk membeli-belah 50X TAE Buffer (Tris-Acetate-EDTA), 1L dalam talian daripada Ubuy.. Anda hanya perlu mencari produk, pilih kaedah penghantaran anda semasa mendaftar keluar dan hantar ke lokasi anda.

Adakah 50X TAE Buffer (Tris-Acetate-EDTA), 1L Tersedia untuk Beli-belah Dalam Talian dalam Malaysia?

Ya, di Ubuy Malaysia produk ini tersedia untuk anda membeli-belah pada harga yang berpatutan.. 50X TAE Buffer (Tris-Acetate-EDTA), 1L tidak tersedia secara tempatan tetapi anda boleh mempercayai kami dengan perkhidmatan penghantaran ekspres kami.

Berapa lama masa yang diperlukan untuk mendapatkan produk selepas membuat pesanan?

Masa penghantaran produk pesanan anda berbeza mengikut apa yang anda pesan dan kaedah penghantaran yang anda pilih.. Anggaran masa penghantaran dinyatakan semasa proses daftar keluar, jadi berhati-hati semasa membeli-belah.

eCon Lab Supply Lihat Asal

50X TAE Buffer: Provides electrical conductivity, inhibits nucleases, and protects DNA during electrophoresis.

eCon Lab Supply

Item #: 66013072

50X TAE Buffer (Tris-Acetate-EDTA), 1L

Item #: 66013072

MYR 590

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from AS}

Ketersediaan : Dalam stok

Diimport dari kedai USA

QTY:

Pesan sekarang dan dapatkannya lebih kurang pada Khamis, Ogos 27

Secured transaction

Our Top Logistics Partners

50X TAE Buffer: Provides electrical conductivity, inhibits nucleases, and protects DNA during electrophoresis.

Waranti U-Care:

Tiada

Pilih pelan

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of AS store (110-120). Recommended power converters Beli Sekarang.

What Stands Out

High-Quality Buffer

This 50X TAE buffer ensures reliable and reproducible performance in nucleic acid experiments, providing an optimal environment for DNA and RNA stabilization during gel electrophoresis.

Concentrated Formula

The 50X concentration allows for easy dilution to meet various experimental needs, minimizing storage space while maximizing efficiency and cost-effectiveness for high-throughput labs.

Versatile Applications

Ideal for diverse applications including agarose gel electrophoresis and molecular biology research, this buffer caters to a broad range of laboratory needs, enhancing its value for various scientific studies.

Maklumat produk

Manufactured in an ISO 9001 and 13485 certified facility in the USA
For research use only. Not for human or animal clinical/therapeutic use.
Manufactured under rigorous quality control tests in a certified clean room environment
Used for running electrophoresis of nucleic acids in gels
EDTA protects DNA from nucleases during electrophoresis
Lower buffering capacity compared to TBE buffer

Container Type	Bottle
Item Form	Gel
Item Weight	1.3 pounds (590 grams)
Volume	1 liters
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.19
Brand	eCon Lab Supply
Item Volume	1 liters
Package Information	Bottle

Who Should Buy?

Molecular Biologists
Ideal for molecular biologists conducting gel electrophoresis on DNA, providing optimal conditions for nucleic acid separation.
Academic Research
Perfect for academic labs performing DNA analysis and cloning, ensuring high integrity of samples during experimentation.
Educational Institutions
Useful for teaching laboratories in schools and universities, facilitating hands-on learning about genetic engineering and biochemistry.

Non-Biological Labs
Not suitable for labs focused on non-biological fields as its applications are specifically for molecular biology and genetics.

PENERANGAN PRODUK

50X TAE Buffer (Tris-Acetate-EDTA), 1L

About This Item

Introducing our 50X TAE Buffer Tris-Acetate-EDTA, a must-have product for all your nucleic acid electrophoresis needs. Our 50X TAE Buffer is specifically designed to provide optimum electrical conductivity and pH maintenance during gel electrophoresis. It contains a high concentration of Tris, Acetic acid, and EDTA to ensure accurate and efficient separation of nucleic acids. Why is this buffer essential? Firstly, the EDTA present in the buffer acts as a powerful inhibitor of metal-dependent nucleases by chelating divalent cations such as Ca2+ and Mg2+.

This protects the integrity of your DNA during the electrophoresis process, giving you reliable and accurate results every time. Not only suitable for genomic and large supercoiled DNA, but our 50X TAE Buffer can also be used as a gel preparation buffer. Its lower buffering capacity, compared to TBE buffer, makes it ideal for applications where faster dsDNA runs are desired. To use our 50X TAE Buffer, simply dilute it with molecular biology grade water to a 1X concentration before running your electrophoresis.

This ensures that the buffer is at the optimal concentration for your experiments. For optimal performance, we recommend periodic replacement of the buffer during prolonged electrophoresis sessions. This helps maintain the buffer's effectiveness and ensures consistent results. So, whether you're a research scientist, a laboratory technician, or a biology enthusiast, our 50X TAE Buffer Tris-Acetate-EDTA is a reliable and essential tool for all your nucleic acid electrophoresis experiments. Invest in the best- buy your 50X TAE Buffer today! Keywords: Tris Acetate EDTA 1L, 50X TAE Buffer 1L,50X TAE Buffer Tris Acetate EDTA, TAE Buffer, Tris Acetate, EDTA.

Ada sebarang Pertanyaan? Berbual dengan kami

Soalan & Jawapan Pelanggan

soalan: Bagaimana untuk Membeli-belah 50X TAE Buffer (Tris-Acetate-EDTA), 1L Dalam Talian Dari Ubuy?

jawab: Sangat mudah untuk membeli-belah 50X TAE Buffer (Tris-Acetate-EDTA), 1L dalam talian daripada Ubuy.. Anda hanya perlu mencari produk, pilih kaedah penghantaran anda semasa mendaftar keluar dan hantar ke lokasi anda.
soalan: Adakah 50X TAE Buffer (Tris-Acetate-EDTA), 1L Tersedia untuk Beli-belah Dalam Talian dalam Malaysia?

jawab: Ya, di Ubuy Malaysia produk ini tersedia untuk anda membeli-belah pada harga yang berpatutan.. 50X TAE Buffer (Tris-Acetate-EDTA), 1L tidak tersedia secara tempatan tetapi anda boleh mempercayai kami dengan perkhidmatan penghantaran ekspres kami.
soalan: Berapa lama masa yang diperlukan untuk mendapatkan produk selepas membuat pesanan?

jawab: Masa penghantaran produk pesanan anda berbeza mengikut apa yang anda pesan dan kaedah penghantaran yang anda pilih.. Anggaran masa penghantaran dinyatakan semasa proses daftar keluar, jadi berhati-hati semasa membeli-belah.

eCon Lab Supply Buffers Editorial Review

The 50X TAE Buffer Tris-Acetate-EDTA is a 1L solution that provides an optimal environment for nucleic acid electrophoresis. It is widely used in molecular biology labs for DNA and RNA analysis. This buffer is prepared with high-quality chemicals and has been proven to deliver consistent and reliable results. The 1L size offers great value for money and ensures that you have an ample supply of buffer for your experiments. Overall, the 50X TAE Buffer Tris-Acetate-EDTA is a highly recommended product for molecular biologists.

Customer Reviews & Ratings

4.0

1 rating pelanggan

5 bintang

0%
4 bintang

100%
3 bintang

0%
2 bintang

0%
1 bintang

0%

Ulas Produk Ini

Kongsikan pandangan anda dengan pelanggan lain

Kebaikan

Optimal environment for nucleic acid electrophoresis
High-quality chemicals
Consistent and reliable results
Great value for money
Ample supply of buffer

Product Price History

Maklumat penting

Pengehadan: Untuk produk penghantaran antarabangsa, harap maklum bahawa sebarang jaminan pengeluar mungkin tidak sah; pilihan perkhidmatan pengeluar mungkin tidak tersedia; manual produk, arahan, dan amaran keselamatan mungkin tidak dalam bahasa negara destinasi; produk-produk (dan bahan-bahan berkaitannya) mungkin tidak direka selaras dengan standard negara destinasi, spesifikasi, dan syarat pelabelan; dan produk-produk itu mungkin tidak mematuhi kepada voltan negara destinasi dan lain-lain standard elektrik (memerlukan penggunaan adaptor atau penukar aliran jika perlu). Penerima adalah bertanggungjawab untuk memastikan bahawa produk itu boleh diimport selaras dengan undang-undang negara destinasi. Apabila memesan dari Ubuy atau pihak afiliasinya, si penerima adalah merupakan pengimport dalam rekod dan mesti mematuhi kepada semua undang-undang dan peraturan negara destinasi.
Bukan semua produk yang tersenarai di Ubuy adalah untuk dijual, kerana Ubuy adalah enjin carian global. Produk-produk adalah tertakluk kepada peraturan eksport/perdagangan.

MYR 590

Pesan sekarang dan dapatkannya lebih kurang pada Khamis, Ogos 27

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

50X TAE Buffer (Tris-Acetate-EDTA), 1L

MYR 590

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Maklumat produk

Who Should Buy?

PENERANGAN PRODUK

About This Item

Soalan & Jawapan Pelanggan

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

Ulas Produk Ini

Kebaikan

Product Price History

Maklumat penting

MYR 590

Ubuy Assurance

Fast
Shipping

Free
Return^*