What is Tris-HCl Buffer and what is its purpose?

Tris-HCl Buffer is a widely used buffering agent that helps maintain a stable pH in biological and chemical experiments. It is particularly effective around a neutral pH of 7.2. The buffer prevents sudden changes in pH, which is critical in experiments involving proteins, nucleic acids, and enzymatic reactions, where even minor pH fluctuations can impact results.

How do I prepare Tris-HCl Buffer at pH 7.2?

To prepare Tris-HCl Buffer at pH 7.2, you will need Tris base and hydrochloric acid (HCl). Start by dissolving the specific amount of Tris base in distilled water and then slowly add HCl while continuously monitoring the pH until you reach the desired level of 7.2. This method allows precise control over the buffer composition, ensuring optimal performance in your experiments.

What applications are suitable for Tris-HCl Buffer, pH 7.2?

Tris-HCl Buffer at pH 7.2 is primarily used in molecular biology applications such as DNA and RNA extraction, gel electrophoresis, and enzyme assays. Its ability to maintain a stable pH makes it a favorite choice in protocols involving sensitive biochemical processes. Additionally, it's commonly utilized in cell culture mediums to ensure optimal growth conditions.

Can I use Tris-HCl Buffer for protein stability studies?

Yes, Tris-HCl Buffer is often utilized in protein stability studies. Its neutral pH environment helps preserve the structure and functionality of proteins, which is essential when investigating protein interactions or enzyme activity. Using this buffer ensures that the proteins remain in an optimal pH range throughout the experiment, thus providing reliable and consistent data.

What is the concentration of Tris in Tris-HCl Buffer, pH 7.2?

In Tris-HCl Buffer, pH 7.2, the concentration of Tris is typically 1M. This indicates the amount of Tris present in the solution, and it is crucial for maintaining the desired buffering capacity. A 1M concentration ensures that even with the addition of acids or bases during experiments, the buffer can effectively stabilize the pH of the solution.

Is Tris-HCl Buffer suitable for use in immunoassays?

Yes, Tris-HCl Buffer, pH 7.2, is suitable for use in immunoassays. Its buffering capacity ensures stable pH levels, which is essential for the performance and specificity of antibodies during assay procedures. This stability allows for consistent results, making Tris-HCl Buffer a preferred choice for various immunological applications.

How should I store Tris-HCl Buffer once opened?

After opening, Tris-HCl Buffer should be stored in a cool, dark place, preferably in a tightly closed container to prevent contamination. Keeping the buffer protected from light and temperature fluctuations will help maintain its quality and effectiveness for future use. Proper storage is key to ensuring the buffer retains its pH stability and overall properties.

Can Tris-HCl Buffer be used in cell culture?

Indeed, Tris-HCl Buffer is commonly utilized in cell culture. It helps maintain the right pH for the growth of various cell lines, providing an optimal environment for cellular functions. This buffer is particularly beneficial in studies involving cellular responses where pH plays a critical role in biochemical pathways and interactions.

What is the shelf life of Tris-HCl Buffer?

The shelf life of Tris-HCl Buffer is generally quite long if stored properly, typically lasting several years. However, it is important to monitor the pH and overall clarity of the solution over time. If any changes are observed in its physical or chemical properties, it may be best to prepare a fresh solution to ensure experimental reliability.

Where can I buy Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml?

You can purchase Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml at Ubuy. They offer a wide variety of laboratory supplies, including biochemicals and reagents like Tris buffers, ensuring you can find the right products for your research needs.

Bagaimana untuk Membeli-belah Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml Dalam Talian Dari Ubuy?

Sangat mudah untuk membeli-belah Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml dalam talian daripada Ubuy.. Anda hanya perlu mencari produk, pilih kaedah penghantaran anda semasa mendaftar keluar dan hantar ke lokasi anda.

Adakah Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml Tersedia untuk Beli-belah Dalam Talian dalam Malaysia?

Ya, di Ubuy Malaysia produk ini tersedia untuk anda membeli-belah pada harga yang berpatutan.. Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml tidak tersedia secara tempatan tetapi anda boleh mempercayai kami dengan perkhidmatan penghantaran ekspres kami.

Berapa lama masa yang diperlukan untuk mendapatkan produk selepas membuat pesanan?

Masa penghantaran produk pesanan anda berbeza mengikut apa yang anda pesan dan kaedah penghantaran yang anda pilih.. Anggaran masa penghantaran dinyatakan semasa proses daftar keluar, jadi berhati-hati semasa membeli-belah.

eCon Lab Supply Lihat Asal

Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification.

eCon Lab Supply

Item #: 94951804

Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml

Item #: 94951804

MYR 457

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from AS}

Ketersediaan : Dalam stok

Diimport dari kedai USA

QTY:

Pesan sekarang dan dapatkannya lebih kurang pada Sunday, Ogos 30

Secured transaction

Our Top Logistics Partners

Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification.

Waranti U-Care:

Tiada

Pilih pelan

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of AS store (110-120). Recommended power converters Beli Sekarang.

What Stands Out

Consistent pH

Offers a stable pH of 7.2, essential for biological experiments and applications, ensuring accurate and reliable results for precise experimental conditions.

High Purity

Made with high-quality reagents that minimize contamination, making it suitable for sensitive assays and research, enhancing the reliability of experimental results.

Convenient Size

Available in a 1000 ml volume, providing ample buffer for extensive laboratory needs, reducing the frequency of repurchase and ensuring long-term usability.

Maklumat produk

Item Form	Liquid
Item Weight	1.5 pounds (680 grams)
Volume	1000 ml
Brand Name	eCon Lab Supply
Manufacturer	Ciro Mfg
Included Components	Bottle
Part Number	23.105.44
Brand	eCon Lab Supply
Item Volume	1000 ml

Who Should Buy?

Biochemistry Labs
Ideal for biochemistry labs conducting experiments requiring stable pH conditions for enzyme reactions and protein studies.
Molecular Biology
Suitable for molecular biology applications, particularly in DNA and RNA extraction protocols needing consistent buffering.
Educational Use
Great for educational institutions conducting practicals to teach students about buffer solutions and biochemical reactions.

Non-scientific Use
Not suitable for individuals or applications outside of scientific research, lacking practical non-laboratory utility.

PENERANGAN PRODUK

Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml

Ada sebarang Pertanyaan? Berbual dengan kami

Soalan & Jawapan Pelanggan

soalan: Bagaimana untuk Membeli-belah Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml Dalam Talian Dari Ubuy?

jawab: Sangat mudah untuk membeli-belah Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml dalam talian daripada Ubuy.. Anda hanya perlu mencari produk, pilih kaedah penghantaran anda semasa mendaftar keluar dan hantar ke lokasi anda.
soalan: Adakah Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml Tersedia untuk Beli-belah Dalam Talian dalam Malaysia?

jawab: Ya, di Ubuy Malaysia produk ini tersedia untuk anda membeli-belah pada harga yang berpatutan.. Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml tidak tersedia secara tempatan tetapi anda boleh mempercayai kami dengan perkhidmatan penghantaran ekspres kami.
soalan: Berapa lama masa yang diperlukan untuk mendapatkan produk selepas membuat pesanan?

jawab: Masa penghantaran produk pesanan anda berbeza mengikut apa yang anda pesan dan kaedah penghantaran yang anda pilih.. Anggaran masa penghantaran dinyatakan semasa proses daftar keluar, jadi berhati-hati semasa membeli-belah.

eCon Lab Supply Buffers Editorial Review

Tiada ulasan editorial ditemui

Customer Reviews & Ratings

4.0

1 rating pelanggan

5 bintang

0%
4 bintang

100%
3 bintang

0%
2 bintang

0%
1 bintang

0%

Ulas Produk Ini

Kongsikan pandangan anda dengan pelanggan lain

Product Price History

Maklumat penting

Pengehadan: Untuk produk penghantaran antarabangsa, harap maklum bahawa sebarang jaminan pengeluar mungkin tidak sah; pilihan perkhidmatan pengeluar mungkin tidak tersedia; manual produk, arahan, dan amaran keselamatan mungkin tidak dalam bahasa negara destinasi; produk-produk (dan bahan-bahan berkaitannya) mungkin tidak direka selaras dengan standard negara destinasi, spesifikasi, dan syarat pelabelan; dan produk-produk itu mungkin tidak mematuhi kepada voltan negara destinasi dan lain-lain standard elektrik (memerlukan penggunaan adaptor atau penukar aliran jika perlu). Penerima adalah bertanggungjawab untuk memastikan bahawa produk itu boleh diimport selaras dengan undang-undang negara destinasi. Apabila memesan dari Ubuy atau pihak afiliasinya, si penerima adalah merupakan pengimport dalam rekod dan mesti mematuhi kepada semua undang-undang dan peraturan negara destinasi.
Bukan semua produk yang tersenarai di Ubuy adalah untuk dijual, kerana Ubuy adalah enjin carian global. Produk-produk adalah tertakluk kepada peraturan eksport/perdagangan.

MYR 457

Pesan sekarang dan dapatkannya lebih kurang pada Sunday, Ogos 30

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

Ciri & Faedah

1M Tris-HCl Buffer, pH 7.2, available in 1000 ml.
Manufactured in an ISO 9001 and 13485 certified facility in the USA.
Produced under stringent Good Manufacturing Practices for superior quality.
Aseptically filtered through a 0.22 micron filter for maximum purity.
Ideal for nucleic acid solutions, including TAE and TBE buffers.
Complete Certificate of Analysis provided for each production lot.

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml

MYR 457

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

Maklumat produk

Who Should Buy?

PENERANGAN PRODUK

Soalan & Jawapan Pelanggan

eCon Lab Supply Buffers Editorial Review

Customer Reviews & Ratings

Ulas Produk Ini

Product Price History

Maklumat penting

MYR 457

Ciri & Faedah

Ubuy Assurance

Fast
Shipping

Free
Return^*