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- Plasmid Miniprep Kit (100)
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Item #:
96773740
Plasmid Miniprep Kit (100)
Item #:
96773740
MYR 692
Price Details
Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )
*All items will import from AS
Dalam stok
Diimport dari kedai USA
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Hanya 7 item ada dalam stok.
Pesan sekarang dan dapatkannya lebih kurang pada Khamis, Julai 23
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High yield, up to 20 µg plasmid DNA can be extracted per prep with a simple and reliable procedure.
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What Stands Out
High Yield
Achieves maximum DNA yield from plasmid isolation, ensuring efficient downstream applications. Ideal for labs requiring concentrated samples for sequencing or cloning.
Quick Protocol
Streamlined 30-minute protocol reduces time spent on plasmid extraction, enhancing workflow efficiency without compromising quality. Perfect for busy research environments.
Kit komprehensif
Includes all necessary reagents and tools for successful plasmid isolation. Tailored for researchers and educators, providing consistent results across various DNA types.
Maklumat produk
- High yield, up to 20 ug plasmid DNA can be extracted per prep
- Simple and reliable procedure
- High purity without contamination
- Cost effective
| Berat item | 1 lbs (450 gram) |
Product Technical Details
| Pengilang | Hygia Reagents |
What's in the box
Plasmid miniprep kit
Who Should Buy?
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Molecular Biologists
Ideal for molecular biologists who need to isolate plasmid DNA quickly and efficiently for research purposes.
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Academic Laboratories
Perfect for academic settings where multiple plasmid extractions are required for experiments, ensuring reliability and reproducibility.
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Genetic Engineers
Suitable for genetic engineers working on cloning, sequencing, or gene expression studies who require high-quality plasmid DNA.
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Hobbyist Labs
Not ideal for hobbyist labs with minimal experience, as the protocol requires precise techniques for successful execution.
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Large Scale Production
Not suitable for large scale plasmid production, as it’s designed for small quantities and may not meet volume needs.
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Non-Biological Fields
Not recommended for users in non-biological fields, as its applications are strictly relevant to molecular biology and genetics.
PENERANGAN PRODUK
Ada sebarang Pertanyaan? Berbual dengan kami
Soalan & Jawapan Pelanggan
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soalan:
What is a Plasmid Miniprep Kit used for?
jawab: A Plasmid Miniprep Kit is specifically designed for isolating plasmid DNA from bacterial cultures. This process allows researchers to obtain pure plasmid DNA for applications such as cloning, sequencing, or transfection into other cells. The kits streamline the purification process, providing reliable yields and high-quality DNA essential for downstream experiments. For instance, if you have transformed E. coli with a plasmid and need to extract it for further gene manipulation, a miniprep kit ensures you achieve this efficiently. -
soalan:
How does the Plasmid Miniprep Kit work?
jawab: The Plasmid Miniprep Kit operates using a column-based method that separates plasmid DNA from cellular debris and genomic DNA. The process typically involves lysing the bacterial cells, neutralizing the lysate, and then passing it through a silica membrane column where plasmid DNA binds while contaminants are washed away. After washing, the plasmid DNA can be eluted in a buffer or water. Scientifically, this method minimizes the risk of contamination and ensures high-quality DNA is accessible for experiments. -
soalan:
How long does the plasmid isolation process take?
jawab: The plasmid isolation process using a Miniprep Kit generally takes around 30 minutes to an hour, depending on the protocol and specific kit used. This efficient timeframe is part of what makes these kits popular among researchers. It allows for quick turnaround in laboratories where time is crucial, such as in cloning projects or when preparing samples for sequencing. Ultimately, the rapid processing ensures that experiments can progress without unnecessary delays. -
soalan:
What volumes of bacterial culture are suitable for the Plasmid Miniprep Kit?
jawab: Most Plasmid Miniprep Kits are optimized for use with bacterial cultures ranging from 1 to 5 mL. The choice of culture volume can impact the yield of plasmid DNA; larger volumes typically yield more DNA. However, it is essential to balance volume with the efficiency of processing to ensure optimal quality. For example, if you are working with a high-copy plasmid, starting with 5 mL culture can significantly enhance your yield, provided you follow the recommended protocols for the kit. -
soalan:
Can the Plasmid Miniprep Kit isolate genomic DNA as well?
jawab: No, the Plasmid Miniprep Kit is specifically tailored for isolating plasmid DNA, not genomic DNA. While both plasmids and genomic DNA exist within bacterial cells, the purification techniques utilized in miniprep kits focus on the smaller, circular plasmids. If your research requires genomic DNA, a different extraction protocol, typically referred to as a Genomic DNA Isolation Kit, should be employed. Understanding this distinction is crucial for researchers to ensure they select the appropriate kit for their specific application. -
soalan:
What factors can influence plasmid yield in a Miniprep?
jawab: Factors that may influence plasmid yield include the growth medium, bacterial strain, and incubation conditions. For example, using a rich medium can promote higher cell density, leading to greater plasmid yield. Additionally, certain bacterial strains are engineered for high plasmid yield, making them preferable for miniprep applications. Monitoring growth conditions, such as temperature and time, can also optimize the yield. For instance, if higher yields are essential for your experiments, consider optimizing your cultivation parameters alongside the miniprep process. -
soalan:
Is additional purification required after using the Plasmid Miniprep Kit?
jawab: In most cases, additional purification steps are not necessary after utilizing a Plasmid Miniprep Kit, especially if the kit is of high quality and designed for DNA applications. However, if you plan to undertake sensitive downstream applications like cloning or sequencing, further purification might enhance results by removing residual contaminants. For example, using techniques such as ethanol precipitation can facilitate even purer plasmid DNA. It's beneficial to consider the end goal of your plasmid manipulation while deciding on the purification level. -
soalan:
What type of plasmid can I isolate with this kit?
jawab: This kit is effective for isolating plasmids of various types, including expression vectors, cloning vectors, and genomic libraries. It is suitable for both high and low-copy plasmids, provided the appropriate protocol is followed. It's crucial to ensure that the plasmid you are working with is compatible with the Miniprep process, as plasmids that are too large or atypical may require specialized extraction methods. Therefore, for standard cloning or expression plasmids, this kit serves as a versatile option. -
soalan:
What reagents are included in the Plasmid Miniprep Kit?
jawab: A Plasmid Miniprep Kit generally includes lysis buffer, neutralization buffer, wash buffer, and an elution buffer. Each of these reagents serves a specific purpose in the DNA purification process. The lysis buffer lyses the bacterial cells to release plasmid DNA, while the neutralization buffer precipitates proteins and genomic DNA. The wash buffer cleanses contaminants away, and the elution buffer aids in retrieving purified plasmid DNA. Understanding the role of each component can help ensure users follow proper protocols effectively. -
soalan:
Where can I buy Plasmid Miniprep Kit 100?
jawab: You can buy the Plasmid Miniprep Kit 100 at Ubuy, which offers a convenient platform for purchasing laboratory equipment and supplies in Malaysia. Ubuy provides access to a variety of scientific products, ensuring that you can find the specific kit you need for your research or academic work.
Hygia Reagents DNA & RNA Extraction Kits Editorial Review
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Customer Reviews & Ratings
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Ulas Produk Ini
Kongsikan pandangan anda dengan pelanggan lain
Kebaikan
- High DNA yield
- Fast and easy to use
- consistent results
- Reliable for plasmid isolation
- Comprehensive protocol included
Keburukan
- Instructions could be clearer.
Platform Trust & Buyer Confidence
MT
Mohd
Verified buyer
“The product received very good packaging & safe…Thank You”
16 June 2026 · via Trustpilot
SJ
Shawati
Verified buyer
“Accurate delivery timing given”
16 June 2026 · via Trustpilot
YB
Youcef
Verified buyer
“Not madly expensive like I thought, and much quicker than promised.”
15 June 2026 · via Trustpilot
LM
Leila
Verified buyer
“Never dealt with Ubuy before, but everything worked out great. Seamless cross border purchasing and shipping. Thanks!”
6/7/2026 · via Trustpilot
KA
Kwame
Verified buyer
“The process was smooth, with clear communication and timelines. This was my 1st purchase and I am really impressed. I will definitely be coming back.”
12 June 2026 · via Trustpilot
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MYR 692
Pesan sekarang dan dapatkannya lebih kurang pada Khamis, Julai 23
This item is not restrict in my country.(Please click on above link if this item is not restrict in your country, So our team will review and allow.)
QTY:
Hanya 7 item ada dalam stok.
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PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.
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Fastest cross-border delivery
Ciri & Faedah
- Extract up to 20 µg plasmid DNA per prep effectively.
- Utilizes a DNA-binding-specific matrix for high efficiency.
- Simple and reliable purification process.
- Removes proteins and cellular components for high purity.
- Cost-effective solution for plasmid purification.
- Ideal for downstream applications like transfection and cloning.
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